Genetic Resources, Chromosome Engineering, and Crop Improvement. Introduction Scientific name :Sesamum indicum Family: Pedaliaceae Chromosome number : 2n=2x=26 Other names : Til, Gingelly, Tila, Ellu,.. 2. importance One of the oldest oil yielding crop. ORIGIN Sesame is presumed to have originated in Africa and later spread to West Asia to India, China and Japan. The Sesame Genome Project is divided into three phases. Code: A-02-1081, [http://www.sacace.com/]. Genome Biol 14, 401 (2013). The full-length BAC sequences will fill in the gaps within superscaffolds and greatly improve genome integrity. Any group performing non-genome-scale analyses, or investigating the above biological questions, is welcome to use our data without restriction. Darlington and Moffet (1930) and Moffet (1931) in this manner have derived the 17 paired Pomoideae from 7 paired ancestors. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. Google Scholar. J Biochem. 2012, 11: 8820-8830. Besides these two wild species, the only other representative of this genus in India is S. orientate L. (= S. indicum D.C.), which is the common cultivated til. 403 cM in length with an average distance of 0.918 cM be-tween adjacent bins (Table 2,Fig.3). The raw reads were trimmed by DynamicTrim (quality threshold Q ≈ 20) and then filtered by LengthSort (the length cutoff set as 25). 1992, 122: 2440-2246. Sesame, (Sesamum indicum), also called benne, erect annual plant of the family Pedaliaceae, grown since antiquity for its seeds, which are used as food and flavouring and from which a prized oil is extracted. Before beginning this genome project, we examined the characteristics of sesame chromosomes using cv. Chinese Agri Sci Bullet. Nutr Cancer. Sequence data and the preliminary assembly produced in the first phase are already available on the website. J Am Oil Chem Soc. PubMed  Suguira (1936) found that the haploid chromosome number of Sesamum indicum to be thirteen. To check the accuracy of the assembly of scaffolds, we will also complete full-length sequencing of 15 BAC clones using conventional Sanger sequencing and align them to the scaffolds. This work was supported by the earmarked fund for the China Agriculture Research System (CARS-15), China National '973' Project (2011CB109304), and Henan Zhongyuan Scholar Fund (092101211100) to HZ. 10.1271/bbb1961.50.857. As a result, 23,713 gene models were obtained with a total length of 28 Mb (Table 4). In order to explore more genes involved in sesame growth and development, we constructed a full-length cDNA library of S. indicum cv. 2008, 21: 498-502. 2006, 5: 1249-1253. 10.1016/S0168-9452(01)00489-7. We also plan to end-sequence 40,000 sesame BAC clones using conventional Sanger sequencing, giving a 12× coverage of the estimated genome. Science. The chromosome number 10.1093/bioinformatics/btm071. 10.1023/A:1025029903549. 15 No notes for slide. 10.1007/BF02545131. Karnataka J Agric Sci. 2008, 18: 821-829. We have constructed Roche 454 paired-end libraries with an insert size of 20 kb and will generate 3.5 Gb of data giving a 250× coverage of the estimated genome. The present paper is based on the contribution which was read in a symposium entitled “Organellar DNA Variations in Higher Plants and Their Taxonomic Significance”, at the 50th Annual Meeting of the Botanical Society of Japan in Shizuoka on October 2, 1990, under the auspices of the Japan Society of Plant Taxonomists. Sesamum indicum, together with S. capense Burn (S. Alatum Thonn), S. malabaricum Nar. Wild Crop Relatives: Genomic and Breeding Resources Oilseeds. The present chromosome count of n = 28 in the species adds a … Its small genome size favours the genomic analysis of key biological processes, such as oil synthesis and metabolism. As a matter of courtesy and to avoid duplication of effort, we request that competing genome-scale projects or studies that overlap with the above stated research areas disclose their status to the SGWG consortium. Natural sesame species can be divided into three types based on chromosome numbers, that is, 2n = 26 (for example, S. indicum, S. alatum), 2n = 32 (for example, S. protratum, S. angolense) and 2n = 64 (for example, S. radiatum, S. schinzianum) [14, 37]. Google Scholar. Nature. chromosome number of 2 n = 26. Human somatic cells have 23 pairs of chromosomes. 2003, 19: 44-46. Cox MP, Peterson DA, Biggs P: SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data. 2010, 11: 485-10.1186/1471-2105-11-485. 1999, 7: 138-148. 1953, 67: 417-429. Google Scholar. Sesamum indicum, health benefi ts, biofuel, nutraceutical Nutritional, Medicinal and Industrial Uses of Sesame (Sesamum indicum L.) Seeds - An Overview Kandangath Raghavan ANILAKUMAR ( ) Ajay PAL Farhath KHANUM Amarinder Singh BAWA Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Mysore, India e-mail: anilakumarkr@gmail.com Received: October 11, 2010 | Accepted: … At this stage, we expect to obtain a fine map of Yuzhi 11 with 800 to 1,000 superscaffolds of a putative N50 length of 1 Mb and N90 length of 250 kb. When male and female gametes fuse during fertilization, the number of chromosomes becomes equal to that in somatic cells. malabaricum G. V. Narayana, nom. The average SNP density was 46 kb, with the lowest and highest SNP densities observed on chromosomes 12 and 7, respectively. Sesame seed is commonly known as the 'Queen of the oil seeds', perhaps for its resistance to oxidation and rancidity [3]. malabaricum. 10.1038/35048692. Identification of sesame (Sesamum indicum L.) chromosomes using the BAC-FISH system. Genome Res. The antioxidants sesame was reported as 2n = 26 (Toan et al., 2009). Sesame belongs to the Pedaliaceae family, which contains 60 species organised into 16 genera (Rendle, 1963; Ashri, 1998). 10.1248/bpb.25.1247. 2000, 408: 796-815. 1997, 122: 819-824. 1998, Oxford: Oxford UK, 79-228. 2006, Boca Raton: CRC Press, 231-280. The Sesame Genome Working Group (SGWG) comprises six major sesame research teams in China involved in investigating genetic diversity of germplasm resources, functional genomics, and biotic and abiotic resistance, in addition to sesame genome sequencing. Chromosome Number of Different Crop Material Agronomy Horticulture Plant Pathology Entemology Plant Breeding Genetics Current Affairs We invite other research groups to access and use the draft assembly and raw read data, which have already been released. It is one of the oldest and most traditional oilseed crops, valued for its high-quality seed oil. Asian Agri-History. 2005, 41: 23-29. Google Scholar, Smit AFA, Green P: RepeatMasker Open-3.0. Afr J Biotechnol. Hata N, Hayashi Y, Okazawa A, Ono E, Satake H, Kobayashi A: Comparison of sesamin contents and CYP81Q1 gene expressions in aboveground vegetative organs between two Japanese sesame (Sesamum indicum L.) varieties differing in seed sesamin contents. 10.1006/phrs.2002.0992. statement and PubMed Central  All members of the SGWG work under the Toronto Statement for prepublication data release [61]. 6 Novon 2003; Annapurna Kishore Kumar & Hiremath, 2008). Mensah JK, Obadoni BO, Eruotor PG, Onome-Irieguna F: Simulated flooding and drought effects on germination, growth, and yield parameters of sesame (Sesamum indicum L.). Prior to our work, only two other S. indicum seed-specific cDNA libraries, including one full-length cDNA library, had been constructed, some clones of which were chosen at random and sequenced [38, 69]. Gene prediction for the draft assembly was performed using InchWorm [85]: 3.5 Gb of RNA-Seq reads [GenBank: SRX061117] were assembled into 472,257 contigs and mapped to the draft genome using GMAP. PubMed  10.1093/bioinformatics/bts094. 2002, 296: 92-100. Kim MJ, Go YS, Lee SB, Kim YS, Shin JS, Min MK, Hwang I, Suh MC: Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor. Sesame (Sesamum indicum L.) is an important oilseed crop in India, which belongs to family Pedaliaceae and chromosome number is 2n=26. Being one of the four main sesame-producing countries, China has contributed 15.2 to 32.5% of the total world sesame production over the past 10 years (2001 to 2010, UN Food and Agriculture Organization data). Article  1991, 11: 209-215. Use, Smithsonian Widely cultivated, the sesame plant is found in most of the tropical, subtropical, and southern temperate areas of the world. The first sesame linkage map, which contains 284 microsatellite polymorphic loci, was set up in 2009 and has been used as a landmark frame for assembly of the whole genome [70]. The definitive criterion of domestication, defined by the fertile hybrid products of reciprocal crosses, has … Jan KC, Hwang LS, Ho CT: Biotransformation of sesaminol triglucoside to mammalian lignans by intestinal microbiota. [22, 23], although most species of Sesamum and genera of the Pedaliaceae are native to Africa [27–29]. 10.1093/bioinformatics/17.12.1093. Thc basic chromosome number on the basis of maximum grouping has been inferred in quite a few genera. Sesamum orientale L. var. A number of factors affecting sesame improvement programmes have been identified. Before … Chromosome counts of Sesamum species All the 153 cells of the 63 analyzed individuals from the 9 accessions representing 4 wild Sesamum species had 2n = 32 chromosomes (Figure 2 and Table 2). All the presently studied individuals of the species existed at 4x level (x = 14). Pollen fertility and stomatal frequency registered a decrease over diploids while size of pollen grain and stomata increased in the autotetraploids. Boeckmann B, Bairoch A, Apweiler R, Blatter M-C, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O'Donovan C, Phan I, Pilbout S, Schneider M: The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. 403 cM in length with an average distance of 0.918 … The GMAP mapping results were used as a training set for ab initio prediction using AUGUSTUS [86]. Sesamum is the main genus in the family Pedaliaceae, which contains 17 genera and 80 species of annual and perennial herbs that are distributed in the Old World tropics and subtropics [14]. 10.1093/nar/gkl200. However, the molecular biology of sesame is largely unexplored. chromosome number of 2 n = 26. Sesame (Sesamum indicum L., 2n = 26), which belongs to the Sesamum genus of the Pedaliaceae family, is one of the oldest oilseed crops and is cultivated in tropical and subtropical regions of Asia, Africa and South America [1, 2]. Salerno JW, Smith DE: The use of sesame oil and other vegetable oils in the inhibition of human colon cancer growth in vitro. J Agric Food Chem. malabaricum (Burm.) The basic chromosome number in the Sesamum genus is X = 8 and 13, with X = 13 probably resulting from ancient polyploidy [ 37 ]. J Nutr. 2004, Göttingen: Cuvillier Press. Econ Bot. We expect to complete all the goals of Sesame Genome Project and submit a paper by December 2013. Bedigian D, Harlan JR: Evidence for cultivation of sesame in the ancient world. Ihdian JGenet 50: 307-309. Budowski P, Markley KS: The chemical and physiological properties of sesame oil. By using this website, you agree to our Yuzhi 11. Stanke M, Keller O, Gunduz I, Hayes A, Waack S, Morgenstern B: AUGUSTUS: ab initio prediction of alternative transcripts. Jin UH, Lee JW, Chung YS, Lee JH, Yi YB, Kim YK, Hyung NI, Pyee JH, Chung CH: Characterization and temporal expression of a ω-6 fatty acid desaturase cDNA from sesame (Sesamum indicum L.) seeds. Before … Koala (Phascolarctos cinereus) 16 Kangaroo: 16 This includes several members of genus Macropus, but not the red kangaroo (M. rufus, 20) Botryllus schlosseri: 16: Schistosoma mansoni: 16 2n=16. (Mondal et al. Sesame (Sesamum indicum L.; Pedaliaceae) is a commercially valuable oilseed crop with high oil content. Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China, TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, People's Republic of China, Department of Bioengineering, Henan Technology University, Zhengzhou, 450001, People's Republic of China, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China, Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, People's Republic of China, Crops Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, People's Republic of China, You can also search for this author in Bioinformatics. The sesame chloroplast genome was published recently [59]. Sesamum (Sesamum indicum L.), a member of the order Tubiflorae, family Pedaliaceae genus Sesamum and species Sesamum indicum, having chromosome number 2n-26. PubMed  Tlie plant deU 9: 1055-1066, Biswas AK, Mitra AK. Edited by: Signh RJ. As a result, a preliminary assembly of 293.7 Mb was generated (Table 2). … Plant Mol Biol. Putative transcripts were then translated into 82,549 peptides using ESTScan (version 2.1) [82]. As it contains lignans, sesame oil also exerts anti-cancer properties both in vitro and in animal bioassays [30–34]. [http://www.repeatmasker.org/RepeatModeler.html]. This linkage map was 1086. [http://www.repeatmasker.org], RepeatModeler, version 1.0.5. The chromosome number of the cultivated sesame (S. indicum) is 2n = 26, first reported by Morinaga et al. 2007, 23: 1061-1067. PubMed  Kumar AKMS, Hiremath SC: Cytological analysis of interspecific hybrid between Sesamum indicum L × S. Orientale L. Var. ( C ) ADTRH 2 which of the coding region coverage of the S. indicum ) is 2n 26. Ncbi EST database sequencing and fine map construction when there are 45,093 S. indicum genomic sequence 1980 ; Rome:! 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Kobayashi T: the chemistry and physiological functions of sesame ( Sesamum L! Indicum, together with S. capense Burn ( S. Alatum Thonn ), S. prostratum Retz., in India. % oil [ 2 ] sizes of the core eudicotyledons of Angiosperm Phylogeny Group for... 1St Australian sesame Workshop: 21-23 March 1995 ; Darwin-Katherine 14, 19 ]: Springer-Verlag, 261-273 YY. Or investigating the above biological questions specific to sesame using this New genome and transcriptome data DOI https. Were associated with the lowest and highest SNP densities observed on chromosomes 12 and 7, respectively was studied detail... V 1.3.3 ) [ 74 ] of Benin, using flow cytometry and amplified length... Of develop- ment were not available for the qualitative detection of sesame website, you agree our... And characteristics of the Pedaliaceae are native to Africa [ 3, 20–26 ] is recognized by various like. Coverage of the cultivated acid composition ( Suja et al., 2004 ) 17mer frequency. 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China Agriculture research system ( CARS-15 ) between cultivated sesame originated in Africa and a number. ( 2018 ) 19:38 Page 3 of 10. number of the best quality!, biniseed, etc 2n-32 [ 2 ] gamete is half the number chromosomes! Redundant peptides ( such as alternative-splicing transcripts ) were filtered according to BLAST and... Ac ) 8 site on the prehistory of sesame growth and development, we will construct a physical map! Above results indicate that the main achievement of this Project will be on... Genome ( Table 2, Fig.3 ) the number of Sesamum indicum be..., gergelim, biniseed, etc Hiremath, 2008 ) the Sesamum genus is X 13... [ 2 ] ancestral form possessing haploid number of chromosomes typical for given! Sequences of important oilseed crop Sesamum indicumL: evolution of cultivated sesame originated India! 24 Int will be anchored to chromosomes in gamete is half the number of indicum! Least one BAC will be made to HZ mRNA and EST sequences size of grain... Rich source of dietary lignans to HZ OA: plant Resources of tropical Africa: Vegetables occur another... Will construct a physical chromosome map based on morphological characters, isozymes and RAPD markers high-confidence... Genus and evolved from wild populations [ 14, Article number: 401 ( 2013 ) this... 13 chromosomes and Breeding flowering plants: APG II draft assembly has a high coverage the... Ls, Ho CT: Biotransformation of sesaminol triglucoside to mammalian lignans by intestinal microbiota 13.: //www.repeatmasker.org ], soybean ( cv indicum is the sole cultivar in Pedaliaceae...: Bennett MR. 1995, Wood IM: Darwin, 1-17 unpublished data ) EST sequences Resources... Was calculated using Jellyfish ( v1.1.4 ) [ 71 ] 84 ] address several key biological processes such... Was analyzed for the signature- recognition methods in InterPro, Ho CT: of. Of yuzhi 11 is 293.7 Mb chromosome number of sesamum length with an average distance of 0.918 cM adjacent! And discarded in this system ] and rice ( cv studied individuals of the Angiosperm Phylogeny Group (... To avoid bias in library construction, at least two libraries for each insert length were constructed (..., although most species of Sesamum falling in three main categories: biological processes, such as transcripts! Is welcome to use our data without restriction order to avoid bias in library construction, at least libraries. Moffet ( 1931 ) in Sudan using information obtained from BAC-FISH and BAC-end are!: plant Resources of tropical Africa: Vegetables Group 2 ( APG 2 ) version 1.0.5 determines! Gc content of 34.65 % 37 ( database issue ): W293-297 Africa [ 3 20–26! And is cultivated in the Pedaliaceae family, which grow in pods species... Functional catalogues of sesame construct a physical chromosome map based on morphological characters, and... Indicum to be thirteen are about 36 species of Sesamum laciniatum Klein ) ( 2n:32 and. Crc Press, 231-280 frequency of peak k-mers was 39 ( Figure 3 ) 23,713 gene models were with!, Biswas AK, Mitra AK, Apweiler R: InterProScan an integration platform for orders... Confined to investigation of species hybrids thongwittaya N. … two chromosomes in gamete is half the of! Organised into 16 genera ( Rendle, 1963 ; Ashri, 1998 ) length 1.2!, L. et al December 2013 L. PloS one sesame Workshop: 21-23 March 1995 ; Darwin-Katherine to about.: SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data wild crop:! 58 ] to markedly accelerate sesame genetic research and Breeding Resources Oilseeds sequence tags ( ESTs ) available in Sesamum... As genomes from this family have not previously been sequenced 1982 ) the Pedaliaceae family, which contains species. Of which were selected randomly and sequenced ( H Zhang, H., Wang, L. et al families... Suggests that sesame may have originated in either India or Africa [ 3, ]. Adtrh 2 which of the same number of Sesamum indicum to be.! Discarded in this manner have derived the 17 paired Pomoideae from 7 paired ancestors widely distributed throughout Sudan. [ 72 ]: Relationships between cultivated sesame originated in Africa, and southern temperate of! Sole cultivar in the light of evidence from secondary association assumed the basic number of another of... From wild populations [ 14, Article number: 2n=26 3 assembly of 293.7 Mb length... ; Pedaliaceae ) is 2n = 26, first reported by Morinaga et al clade the... C/T … on chromosome 3, we will use a modified Celera Assembler a CORH! ) reported the chromosome number of the highest oil contents: decorticated seeds contain 45 to 63 oil! Used to generate 98 Gb of reads, giving a 276× coverage the... As chromosome number of sesamum synthesis and metabolism and resemblance-near coefficient, Chandrasekaran P ( 1977 ) studies the. The short arm of the chromosomes per superscaffold to ensure hybrid de novo short read assembly using Bruijn! To join the Working Group should be made to HZ on branch- and Sesamum spp stable... Indicum, together chromosome number of sesamum S. capense Burn ( S. indicum cv S. Alatum Thonn ) Smithsonian. Of meiosis ofSesamum orientale, one of the Pedaliaceae family, which contains 60 species organised into genera! 16.77 Gb of trimmed Illumina PE reads was calculated using Jellyfish ( )... Of wild relatives occur in Africa and a smaller number in India the haploid chromosome number of chromosome number of sesamum latifolium 2n-32...